I.L. Correction

In immunofluorescence experiments there is a common problem where images taken from an optical microscope contain overexposure. One of the biology labs at the University of Montana was running immunofluorescence experiments and were correcting the overexposure by hand. This project was designed to alleviate this manual process by parsing a directory of images and automatically correcting the overexposure.

The code can be downloaded an ran from its GitHub Repo.

Examples of Light Correction

The following images are the before and after pictures of immunofluorescence experiments ran through this software application

immunofluorescence photo of a set of blue-dyed cells before light correction

immunofluorescence photo of a set of blue-dyed cells after light correction

immunofluorescence photo of a set of green-dyed cells before light correction

immunofluorescence photo of a set of green-dyed cells after light correction

immunofluorescence photo of a set of red-dyed cells before light correction

immunofluorescence photo of a set of red-dyed cells after light correction